Advanced Lameness Technologies 

CenTrate™ BMA Device

Bone Marrow Aspirate Concentration

CenTrate is a proprietary bone marrow aspirate concentration device whose output produces a natural suspension of plasma proteins. 

Note: In addition to ACD-A, Heparin is also an appropriate anti-coagulant when processing the CenTrate device. See instructions for concentration guidelines.

Features of the CenTrate Device include:

• Dual buoy design eliminates need to filter bone marrow prior to processing

• 5-19x increase in MSCs compared to original bone marrow 

• Natural fibrin matrix

• 15-minute processing

Bone marrow is often aspirated to utilize the stem cells for tissue repair applications.

Bone marrow aspirate (BMA) is a rich source of MSCs and other osteoprogenitor cells in the body.

The Role of Bone Marrow: The role of bone marrow in the body is to create blood and the

vascular system. When bone marrow cells concentrate at an injury site they initiate new blood vessel formation to start the next phase of the healing process.

Bone Marrow Aspirate Concentrate
The CenTrate Bone Marrow Aspirate Concentration device efficiently concentrates cells and platelets in a natural suspension of plasma proteins in 15 minutes. The dual buoy design eliminates the need to filter bone marrow prior to processing. 

Contains Cells and Cytokines

• Mesenchymal stem cells (MSCs),2 endothelial progenitor cells (EPCs),3 and hematopoietic stem cells (HSCs).4

• High concentrations of interleukin-1 receptor antagonist (IL-1ra or IRAP)5 and tissue healing growth factors.6 

Figure 1: Schematic representation of a bone marrow aspiration to acquire cells from blood and bone marrow. Blood contains platelets, white blood cells, and red blood cells. Bone marrow contains white blood cells and musculoskeletal progenitor cells. 

The bone marrow aspirate (BMA) needle (sold separately) used with the CenTrate Device

Six holes at the distal tip for better aspiration

3cc or 6cc of cell-rich solution

Each needle comes with a trocar point and blunt tip

What's needed to process and administer the CenTrate 60ml Device...

• Owl Manor Centrifuge

• Green (60 ml) Centrifuge Buckets

• Blue (60 ml) Counterbalance - Adjustable or Solid

The items below are not included:

For bone marrow harvest (Heparin or ACD-A):

• Anti-coagulant - Heparin or ACD-A

• (1 or 2) 60-ml syringe (Luer-lock recommended) to harvest marrow

For Step 1 (following 1st spin)

• (1) 30-ml syringe to draw / remove Platelet Poor Plasma 

• (1) 10-ml (Luer-lock recommended) syringe to draw out BMA concentrate

CenTrate Device in sterile packaging

CenTrate Device and Blue 60 ml Counterbalance

CenTrate Device and Blue 60 ml Counterbalance in Green Centrifuge Buckets 

References

1, Imam, M.A.; Mahmoud, S.S.S.; Holton J.; Abouelmaati, D.; Elsherbini, Y.; Snow, M., A systemic review of the concept and clinical applications of Bone Marrow Aspirate Concentrate in Orthopaedics. SICOT J 2017, 3(17).

2. Pittenger, M. F.; Mackay, A. M.; Beck, S. C.; Jaiswal, R. K.; Douglas, R.; Mosca, J. D.; Moorman, M. A.; Simonetti, D. W.; Craig, S.; Marshak, D. R., Multilineage potential of adult human mesenchymal stem cells. science 1999, 284 (5411), 143-147.

3. Khakoo, A. Y.; Finkel, T., Endothelial progenitor cells. Annu. Rev. Med. 2005, 56, 79-101.

4. Méndez-Ferrer, S.; Michurina, T. V.; Ferraro, F.; Mazloom, A. R.; MacArthur, B. D.; Lira, S. A.; Scadden, D. T.; Ma’ayan, A.; Enikolopov, G. N.; Frenette, P. S., Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. nature 2010, 466 (7308), 829.

5. Jordan, M.; Otterness, I. G.; Ng, R.; Gessner, A.; Röllinghoff, M.; Beuscher, H., Neutralization of endogenous IL-6 suppresses induction of IL-1 receptor antagonist. The Journal of Immunology 1995, 154 (8), 4081-4090.

6. King, W.; Tan, M.; Ponticiello, M.; Woodell-May, J., Anti-Inflammatory Properties of the Output of an Autologous Bone Marrow Concentrating Device. Orthopedic Research Society Annual Meeting 2016.